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Tufts Equine Respiratory Health Lab

Bronchoalveolar Lavage (BAL)

The bronchoalveolar lavage (BAL) allows us to see deep within the horse’s lungs to determine if there is inflammation and if so, what the inflammation looks like. The BAL is sometimes called a lung wash because we literally wash the smallest airways and alveoli (tiny lung sacs where oxygen is exchanged) with sterile saline. When we suction back that fluid, we bring cells and mucus out of the lung, and then we make a cytology of that fluid (a slide that we can look at under the microscope). The BAL can be done blindly in the field with a specialized silicone tube, called a Bivona BAL tube. This is a very useful technique and is practical for ambulatory veterinarians. At Cummings, we prefer to do a full bronchoscopy. This allows us to examine all the airways as we obtain the BAL, and it also allows us to examine the upper airway to determine if there are any abnormalities that may be contributing to a horse’s cough or nasal discharge in addition to IAD.

Clients and referring veterinarians sometimes ask us why we don’t just do a tracheal aspirate using a regular endoscope, which is a very simple procedure and doesn’t require any specialized equipment. The answer is that this method takes cells from what we term the ‘tracheal puddle’ toward the end of the trachea, and multiple studies have shown that what we see in the tracheal puddle does not reflect what we see in the BAL. Quite simply, the two methods are not equivalent, and the tracheal aspirate is inferior to the BAL if we want to know what is going on in the small airways, which is what we care about with IAD horses.

BAL Technique

The bronchoalveolar lavage is best used in cases where we suspect that the disease we are looking for is diffuse, and when we are not planning to culture the fluid. It is most commonly used for suspected IAD or heaves, but can also be useful for interstitial pneumonia, fungal pneumonia or silicosis, for example.

Your veterinarian will listen to your horse’s heart with a stethoscope before performing the BAL. Although the BAL is common and safe, we prefer not to perform it on a horse with cardiac arrhythmia or a very high respiratory rate. Your veterinarian will also warn you that your horse is likely to cough a lot during the procedure and while it may sound alarming, it should be expected. Before performing the BAL, your veterinarian will heavily sedate your horse. There are several reasons why heavy sedation is essential: firstly, we are aware that when people experience a BAL, they don't feel pain, but they do report feeling panicky. We want to sedate the horse to keep it from feeling any unpleasant sensations; secondly, we need the tube to go into the horse’s trachea and not the esophagus, so we have to stretch the head out and encourage the horse to leave the airway open; and finally, we want to be able to wedge the tube or endoscope firmly in the lower airway so that we get a true picture of the cells in that area. It will look to you as though your veterinarian is using a lot of sterile fluid to wash the lungs – and it is a lot. We use half a liter of sterile fluid to do a BAL. However, your horse’s lungs are enormous and any fluid that is not suctioned back is rapidly absorbed by the great veins in the lungs, so there is no risk of drowning your horse, as clients sometimes fear. By using a large, standard amount of fluid, we make sure that we get repeatable results that can be compared to known standards – this is critical for a correct diagnosis. Once your veterinarian has instilled and suctioned back the full 500mls of fluid, they will place it on ice to keep the cells happy until they are back at the clinic where slides can be made or the fluid can be sent to our laboratory for analysis.

BAL Cytology

Horses normally have a scant amount of mucus in their lower airways, along with alveolar macrophages, lymphocytes and a scant number of neutrophils, mast cells and eosinophil.

Alveolar macrophages are the predominant cells in the airways of most mammals, including humans, but they only account for approximately 60% of the total cell population in horses; lymphocytes are the other heavy hitters, accounting for about 40% of the total cell population in the lungs. Normal horses have less than 5% neutrophils, less than 2% mast cells and less than 0.5 % eosinophils. Let’s talk for a minute about what these cells do.

We think of alveolar macrophages as the normal cells of the lung. Alveolar macrophages are the ‘clean-up’ cells that are part of the innate immune system. They recognize particulates that don’t belong in the lung and get rid of those particulates, including bacteria, before they can cause damage. In the picture here

a spore from one of the many fungi that are found in even the best hay

A spore from one of the many fungi that are found in even the best hay

you can see one of the worst offenders in equine lungs – a spore from one of the many fungi that are found in even the best hay. This is not an infection, but more like a foreign body to which the horse’s lungs are mounting an inflammatory response. Lymphocytes are rare in the lower lungs of most species other than horses. They are important to immune function but have an important role in the adaptive immune system, which the body uses to recognize specific pathogens and have what is termed ‘memory’. Neutrophils are scarce in the healthy lung of any species, but proliferate in horses with heaves/RAO and also more severe or chronic IAD.

Staining purple granules of mast cells associated with respiratory allergies

Staining purple granules of mast cells associated with respiratory allergies

Mast cells are important in allergic diseases, and although their staining characteristics are very aesthetically pleasing, the beautifully staining purple granules are the very things that cause many of the symptoms associated with respiratory allergies, including bronchospasm and cough. Eosinophils are rare in the healthy horse but can be seen more frequently in the BALF in the summertime, or in horses that have intestinal parasites. Less commonly, we see goblet cells, which produce mucus and both ciliated and non-ciliated epithelial cells. Epithelial cells should be rare to absent in the normal BALF: we see them more frequently in horses with cough or who have had viral respiratory disease in the recent past.

goblet cells

Goblet cells, which produce mucus and both ciliated and non-ciliated epithelial cells

Although some laboratories suggest straining BALF to remove mucus, our laboratory prefers not to, as microscopic analysis of the mucus itself can be important to the diagnosis of disease, and mucus often has trapped particulates such as pollen and mold spores, allowing the cytologist to detect the original problem.

Ready to find out more?

Download Dr. Mazan's Equine Respiratory Health e-book for a detailed understanding of equine respiratory health.

Our Doctors

  • Dr. Daniela Bedenice

    Daniela Bedenice

    Dr. Med Vet, DACVIM, DACVECC, Section Chief

  • Dr. Melissa Mazan

    Melissa Mazan


Dr. Daniela Bedenice

Daniela Bedenice

Dr. Med Vet, DACVIM, DACVECC, Section Chief

Dr. Daniela Bedenice is a veterinary internist, Section Chief and one of the few large animal veterinary specialists in the country who is dual board-certified in both Large Animal Internal Medicine and Emergency and Critical Care. Dr. Bedenice co-leads the Tufts Equine Respiratory Lab and is at the forefront of understanding, diagnosing and treating horses with respiratory conditions. She also provides intensive care for both newborn and adult horses, llamas, alpacas and small ruminant species. Dr. Bedenice grew up in rural Germany where she trained and cared for many horses. After earning her veterinary degree from the Free University of Berlin, Dr. Bedenice initially worked in private practice followed by specialty training in large animal internal medicine, critical care and respiratory medicine after moving to the United States. As an Associate Professor in the Department of Clinical Sciences at Cummings School, Dr. Bedenice has published extensively in the field of comparative respiratory medicine and evidence-based therapy, as well as newborn and adult intensive care. She is Course Director of Clinical Pharmacology and leads or contributes to multiple veterinary courses focused on respiratory topics, gastrointestinal disease and neurology, and to clinical services specializing in large animal internal medicine. At home, Dr. Bedenice operates a small farm with thirteen alpacas and three German Shepherds.

Internal Medicine Service

Dr. Melissa Mazan

Melissa Mazan


Dr. Melissa Mazan co-leads the Tufts Equine Respiratory Lab and is a world-recognized expert in the field of equine asthma. Dr Mazan’s work led to the creation of the first clinical lung function laboratory in North America where lung function testing is regularly used to help improve equine performance. Dr. Mazan earned her B.A. at Yale University and her DVM from Cummings School of Veterinary Medicine at Tufts University.  She is board-certified in the America College of Veterinary Internal Medicine (Large Animal). Dr. Mazan teaches courses on respiratory medicine and pathophysiology, as well as exercise physiology at Cummings School where she was drawn by the opportunity to combine research, clinical work and teaching.  She also developed the first veterinary telemedicine teaching service in the United States for working equids in Fez, Morocco. Dr. Mazan’s interest in equine health and performance began when she was a member of the Varsity Polo Team at Yale University and the Captain of the Polo Team at the University of Oxford, and when she was managing her own barn and competing as a junior in equitation and Pony Club.

Internal Medicine Service

BAL Cytology Service

When we do a BAL cytology at the Tufts Equine Respiratory Health Lab,  you not only get a cytological diagnosis, but you also have access to our clinicians for advice on treatment.  Find out more about our service and how to submit samples.